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| Images of tissue total RNA used for construction of Northern blots and analysis with cDNA probes. Click on each image for enlargment | |||
Zyagen offer Northern blots made of a wide variety of Sprague Dawley rat tissues (neuronal, digestive, reproductive, circulatory, respiratory, urinary, immune, and mixed tissue panels). Blots are made of high quality total RNA samples which are subjected to rigorous quality control procedures before fractionation through large denatured agarose gels and blotting onto positively charged nylon membranes. .
Blots can be used with either radioactive or nonisotopic-labeled probes to:
- Quickly assess gene expression across rat tissues.
- Determine size and relative abundance of transcripts in different tissues.
- Identify alternative splice forms.
Benefits:
- Ready-to-hybridize Northern blots of total RNA of tissues that are difficult to obtain because of size, high RNase content, and anatomical complexity which requires knowledge, experience, and technical skill to exactly dissect the area of interest freely of surrounding tissues.
- Saving labor by eliminating tedious and time-consuming laboratory procedures (harvesting and homogenization of tissues, RNA extraction, RNA quantification, gel electrophoresis, and blotting).
- High standards of quality control
- Economic, using multiple tissues at a cost much lower than it would take to do in your laboratory. Think to the labor and cost of laboratory materials, supplies and animals (purchasing, transportation, housing, disposable of carcasses and waste materials).
- Reusable for several times, just strip probe and re-hybridize with new one.
Quality control:
Every step of preparation of blots, from harvesting tissues and extraction of RNA to the blotting, is carefully monitored to ensure the superior quality and performance.
- Blots are made of total RNA that is treated with RNase-free DNase to remove residual DNA. The purity and integrity of RNA are tested by denaturing agarose gel electrophoresis.
- The efficiency of transfer is checked by staining gels with reversible staining .
- The integrity of the blotted RNA is tested by beta-actin specific probe.
Description: The freshly isolated rat tissue total RNA samples (20ug each) are fractionated though large formaldehyde-denatured agarose gel eletrophoresis, blotted onto a positively charged nylon membranes, and immobilized by UV light. Blots are shipped in a sealed bag at room temperature and are good for 1 year if stored at 4oC in the sealed bags.
Custom Services: If you are interested in Northern blot constructed with any rat RNA tissue of any strain or required blots to be specifically prepared to meet your application, our custom service scientists can make it for you. For more information, contact technical support at zinfo@zyagen.com or at 858 546 0720.
Rat Tissue Northern Blots:
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Lane #
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Panel# 1
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Panel# 2
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Panel# 3
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Panel# 4
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Panel# 5
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Panel# 6
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Panel# 7
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Panel# 8
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Panel# 9
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Premade NB, Major Tissues
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Premade NB, Minor Tissues
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Premade NB, Neuronal Tissues
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Premade NB, Digestive Tissues
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Premade NB, Reproductive Tissues
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Premade NB, Circulatory, Respiratory, Urinary, Immune Tissues
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Custom Made NB, any 10 tissues of only Panel# 1
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Custom Made NB, any 10 tissues of Panel# 1 to 6
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Custom Made NB, any 15 tissues of Panel# 1 to 6
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RN-MT-1
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RN-MT-2
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RN-MT-3
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RN-MT-4
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RN-MT-5
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RN-MT-6
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RN-CUS-1
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RN-CUS-2
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RN-CUS-3
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M
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RNA Marker
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RNA Marker
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RNA Marker
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RNA Marker
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RNA Marker
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RNA Marker
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RNA Marker
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RNA Marker
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RNA Marker
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1
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Brain
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Pituitary
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Brain, whole
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Esophagus
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Ovary
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Heart
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Tissue 1
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Tissue 1
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Tissue 1
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2
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Liver
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Thyroid
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Cerebellum
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Stomach
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Uterus
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Atrium
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Tissue 2
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Tissue 2
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Tissue 2
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3
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Pancreas
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Adrenal
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Hippocampus
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Duodenum
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Cervix
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Ventricle
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Tissue 3
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Tissue 3
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Tissue 3
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| 4 | Spleen | Lymph Nodes | Hypthalumus | Jejunum | Vagina | Aorta | Tissue 4 | Tissue 4 | Tissue 4 |
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5
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Heart
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Eye
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Thalamus
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Ileum
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Testis
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Trachea
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Tissue 5
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Tissue 5
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Tissue 5
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6
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Lung
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Adipose
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Striatum
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Colon
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Epididymis
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Lung
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Tissue 6
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Tissue 6
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Tissue 6
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7
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Esophagus
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Bone Marrow
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Medulla
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Cecum
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S. Vesicles
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Kidney
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Tissue 7
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Tissue 7
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Tissue 7
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8
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Stomach
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Bone
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Pons
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Rectum
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Vas Deferens
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Bladder
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Tissue 8
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Tissue 8
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Tissue 8
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9
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Intestine
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Blood cells
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Cerebral Cortex
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Liver
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Prostate
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Thymus
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Tissue 9
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Tissue 9
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Tissue 9
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10
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Colon
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Olfactory Bulb
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Pancreas
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Placenta
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Spleen
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Tissue 10
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Tissue 10
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Tissue 10
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11
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S. Muscles
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Spinal Cord
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Salivary
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Mammary
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Lymph Nodes
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Tissue 11
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12
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Kidney
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Tissue 12
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13
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Thymus
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Tissue 13
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14
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Testis
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Tissue 14
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15
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Prostate
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Tissue 15
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16
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Ovary
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17
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Placenta
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18
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Mammary
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19
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Skin
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