Rat developmental tissue Western blots are made of high quality total proteins that are extracted from several tissues of Sprague Dawley rats at different stages of life and subjected to rigorous quality control procedures before fractionation through large SDS-PAGE.
Blots can be used to:
- Quickly assess protein expression across rat tissues at different stages of development.
- Determine size and relative abundance of transcripts in developmental tissues.
- Identify alternative splice forms.
Benefits:
- Ready-to-use Western blots from high quality tissue lysates that are difficult to obtain because of size, high-protease tissue content, or anatomical complexity of the tissues which requires knowledge, experience, and technical skill to dissect the areas of interest freely of any surrounding tissues.
- Saving labor by eliminating tedious and time-consuming laboratory procedures (harvesting and homogenization of tissues, protein extraction, quantification, gel electrophoresis, and blotting).
- High standards of quality control
- Economic, using multiple tissues at a cost much lower than it would take to do in your laboratory. Think to labor and cost of laboratory materials, supplies and animals (purchasing, transportation, housing, disposable of carcasses and waste materials).
- Reusable for at least two times, just strip and use with other antibody.
Quality control:
Every step of preparation of blots, from harvesting tissues and extraction of protein to blotting is carefully monitored to ensure the superior quality and performance.
- Blots are made from high quality tissue lysates that is homogenized in a protein lysis buffer containing cocktail of 7 mammalian protease inhibitors. The quality of protein as indicated by the absence of smear (no degradation) and sharpness and resolution of protein bands are verified by denatured SDS-PAGE with Coomassie blue staining.
- The efficiency of transfer is routinely checked by staining protein on membranes with reversible Ponceau staining.
- The integrity of the blotted protein is tested by immunostaining of b-actin.
Description:
The total protein samples (75ug each ) are freshly extracted from tissues, boiled in SDS sample buffer, fractionated though 12.5% SDS-denaturing large polyacralamide gel eletrophoresis (SDS PAGE), and blotted onto PVDF membranes. Each blot contains protein samples plus one prestained protein molecular weight standards. Each blot is shipped in a sealed bag at room temperature and is good for one year if properly stored dry at 4oC in a sealed bag with descant.
Custom Services: Zyagen also offers custom services to make ready-to-use Western blots constructed with total proteins of any developmental tissues of any rat strains, contact technical support at zinfo@zyagen.com or at 858 546 0720 for more information.
Rat Developmental Tissue Western Blots
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Lane#ts
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Brain Develop Western Blot |
Heart Develop Western Blot |
S Muscles Develop Western Blot | Intestine Develop Western Blot | Ovary Develop Western Blot | Testis Develop Western Blot | Mammary Develop Western Blot |
| Cat.#: RW-201-D | Cat.#RW-801-D | Cat.#: RW-102-D | Cat.#RW-306-D | Cat.#: RW-406-D | Cat.#RW-401-D | Cat.#: RW-414-D | |
| M | Protein Marker | Protein Marker | Protein Marker | Protein Marker | Protein Marker |
Protein Marker
|
Protein Marker |
| 1 | E20 | E20 | E20 | E20 | 2 Weeks old | 2 Weeks old | Non-Pregnant |
| 2 | 1 Day old | 1 Day old | 1 Day old | 1 Day old | 3 Weeks old | 3 Weeks old | E12 |
| 3 | 1 Week old | 1 Week old | 1 Week old | 1 Week old | 1 Month old | 1 Month old | E14 |
| 4 | 2 Weeks old | 2 Weeks old | 2 Weeks old | 2 Weeks old | 2 Month old | 2 Month old | E16 |
| 5 | 3 Weeks old | 3 Weeks old | 3 Weeks old | 3 Weeks old | 3 Months old | 3 Months old | E18 |
| 6 | 1 Month old | 1 Month old | 1 Month old | 1 Month old | 6 Month old | 6 Month old | E20 |
| 7 | 2 Months old | 2 Months old | 2 Months old | 2 Months old | 12 Months old | 12 Months old | Day1 of Lactation |
| 8 | 3 Months old | 3 Months old | 3 Months old | 3 Months old | Day3 of Lactation | ||
| 9 | 6 Months old | 6 Months old | 6 Months old | 6 Months old | Day7 of Lactation | ||
| 10 | 12 Months old | 12 Months old | 12 Months old | 12 Months old | Day1 of Involution | ||
| 11 |
|
|
|
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Day7 of Involution |