Panel 6: Mouse CD1 Cir, Res, Uri, Imm Tissue Premade Northern Blot

$495.00

Mouse CD1 Circulatory, respiratory, urinary, and immune tissue Northern blot is made of total RNA extracted from 11 tissues of CD1 (ICR) mouse strain (Panel# 6). Total RNA samples are subjected to rigorous quality control procedures before fractionation through large denatured agarose gels. Blots can be used with either radioactive or nonisotopic-labeled probes to:
  • Quickly assess gene expression across mouse tissues.
  • Determine size and relative abundance of transcripts in different tissues.
  • Identify alternative splice forms.
Quality control:
Every step of preparation of blots, from harvesting tissues and extraction of RNA to the blotting, is carefully monitored to ensure the superior quality and performance.
  • Blots are made of total RNA that is treated with RNase-free DNase to remove residual DNA. The purity and integrity of RNA are tested by denaturing agarose gel electrophoresis.
  • The efficiency of transfer is checked by staining gels with reversible staining .
  • The integrity of the blotted RNA is tested by beta-actin specific probe.
Description: The freshly isolated total RNA samples (20ug each) plus one RNA marker are fractionated through large formaldehyde-denatured agarose gel eletrophoresis, blotted onto a positively charged nylon membranes, and immobilized by UV light. Blot is shipped in a sealed bag at room temperature and is good for 1 year if properly stored at 4oC in the sealed bag.

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