Guinea Pig Testis full length tissue cDNA is made of RNA extracted from freshly harvested tissues of single healthy normal donor using classical guanidine isothiocyanate-phenol:chloroform extraction method. RNA is treated with RNase-free DNase-1 to remove residual DNA. The cDNA is primed with oligo dT primer.
Quality Control: The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by spectrophotometer (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR.
The synthesized cDNA is also tested as template for PCR amplification of ß-actin gene. PCR product of ß-actin was visualized on 1% agarose gel.
Applications: cDNA is ideal for gene expression analysis by PCR, characterization of alternative splicing of mRNA, verification of genetic mutation, gene cloning and target sequencing.
Packing/shipping: each cDNA sample is routinely shipped on dry ice in 1.5 ml vials and is enough for 30 reactions (30 PCR amplifications).
MSDS and Certificate of Analysis in PDF files: Contact Zyagen Technical Support at firstname.lastname@example.org