Mouse testis total RNA is pure intact total RNA extracted from mouse whole testis tissues using classical guanidine thiocyanate/phenol:chloroform extraction method so large and microRNA are included in sample. Total RNA sample is treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80oC. The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by spectrophotometer (A260/A280: 1.9-2.1).
RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR analysis, rapid amplification of cDNA ends (RACE) and purification of mRNA for library construction.
Total RNA is provided in RNase-free water, 1mM sodium citrate, or 0.1 mM EDTA at a concentration of 1 mg/ml and shipped on dry ice.
