Bcl-G Rabbit Polyclonal Antibody

$315.00

APO-3165

Background

Bcl-G Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bcl-G. The mRNA of Bcl-G encodes 2 isoforms, Bcl-GL, which is widely expressed in multiple tissues, and Bcl-GS, which is only found in testis. The Bcl-GS protein is predominantly localized to cytoplasmic organelles whereas Bcl-GL was distributed throughout the cytosol. Overexpression of either protein induced apoptosis, although Bcl-GS was far more potent than Bcl-GS. Apoptosis induction was dependent on the BH3 domain and could be suppressed by co-expression with the anti-apoptotic Bcl-XL protein.

 

Description
Bcl-G Antibody
Western blot analysis of Bcl-G in U937 cell lysates with Bcl-G antibody at (A) 2.5 and (B) 5 μg/mL.
Bcl-G Antibody
Immunohistochemical staining of mouse testis tissue using Bcl-G antibody at 2 μg/mL.

 

 

 

 

 

 

 

 

 

 

 

 

 

Specifications

Additional Names
Bcl-G Antibody: Bcl-2-like 14
User Note
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgG
Specificity
Although antibody should react with both isoforms, only the Bcl-GS protein has been observed
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid 

Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal Bcl-G Antibody.
Purification
Bcl-G Antibody is Ion exchange chromatography purified.
Host
Bcl-G antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.
Immunogen
Bcl-G antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human Bcl-G.
Application
Bcl-G antibody can be used for detection of Bcl-G by Western blot at 2.5 to 5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Tested Application
E, WB, IHC, IF
Buffer
Bcl-G Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. ZP-3165 - Bcl-G Peptide
Storage
Bcl-G antibody can be stored at 4ËšC for three months and -20ËšC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Species Reactivity
H, M, R
Protein GI Number
13540528
Protein Accession Number
NM_030766
Reference

  • Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.
  • Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron.. 2004; 39:1125-35.
  • Guo B, Godzik A, and Reed JC. Bcl-G, a novel pro-apoptotic member of the Bcl-2 family. J. Biol. Chem. 2000; 276:2780-5.]

 


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