Bcl-B Rabbit Polyclonal Antibody

$315.00

APO-3163

Background

Bcl-B Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-B is a recently discovered anti-apoptotic member of the Bcl-2. Unlike the mouse homolog (also known as Diva/Boo) which is predominantly expressed in ovary and testis, the human Bcl-B protein is widely expressed. Also, the human Bcl-B functions by binding to and suppressing the apoptotic activity of Bax, whereas the mouse homolog binds Bak and also interacts with the apoptosis protein Apaf-1.

 

Description
Bcl-B Antibody
Western blot analysis of Bcl-B in Jurkat lysate with Bcl-B antibody at 1 μg/ml in the (A) absence and (B) presence of blocking peptide.
Bcl-B Antibody
Immunocytochemistry of Bcl-B in Jurkat cells with Bcl-B antibody at 10 μg/mL.

 

 

 

 

 

 

 

 

 

Specifications

Additional Names
Bcl-B Antibody: Boo, Diva, Nrh
User Note
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgG
Immunogen
Bcl-B antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human Bcl-B.
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid 

Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal Bcl-B Antibody.
Purification
Bcl-B Antibody is affinity chromatography purified via peptide column.
Host
Bcl-B antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.
Application
Bcl-B antibody can be used for the detection of Bcl-B by Western blot at 1 μg/mL. Despite its predicted molecular weight, Bcl-B is often at higher molecular weights, presumably due to post-translational modifications. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.
Tested Application
E, WB, ICC
Buffer
Bcl-B Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. ZP-3163 - Bcl-B Peptide
Storage
Bcl-B antibody can be stored at 4ËšC for three months and -20ËšC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Species Reactivity
H
Protein GI Number
9966783
Protein Accession Number
NP_065129
Reference

  • Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607
  • Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron. 2004; 39:1125-35.
  • Ke N, Godzik A, and Reed JC. Bcl-B: A novel Bcl-2 family member that differentially binds and regulates Bax and Bak. J. Biol. Chem. 2001; 276:12481-4.
  • Song Q, Kuang Y, Dixit VM, et al. Boo, a negative regulator of cell death, interacts with Apaf-1. EMBO J. 1999; 18:167-78.]

 


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