Bcl-2 Rabbit Polyclonal Antibody

$315.00

APO-3335

Background

Bcl-2 Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. Bcl-2 is the founding member of a family of over 20 proteins that are critical regulators of apoptosis. These can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bcl-2 is an inner mitochondrial membrane protein that inhibits apoptosis. It is thought to act by interacting with pro-apoptotic Bcl-2 family members such as Bak and Bad. Overexpression of Bcl-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.

 

Description
Bcl-2 Antibody
Western blot analysis of Bcl-2 in Daudi cell lysates with Bcl-2 antibody at (A) 1, (B) 2, and (C) 4 μg/mL.
Bcl-2 Antibody
Immunohistochemical staining of human kidney using Bcl-2 antibody at 2 μg/mL.

 

 

 

 

 

 

 

 

 

 

 

Specifications

Additional Names
Bcl-2 (NT), Bcl-2
Additional Names
Bcl-2 Antibody:
User Note
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgG
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid 

Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal Bcl-2 Antibody.
Purification
Bcl-2 Antibody is Ion exchange chromatography purified.
Host
Bcl-2 antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.
Immunogen
Bcl-2 antibody was raised against a peptide corresponding to 15 amino acids near the N terminus of human Bcl-2.
Application
Bcl-2 antibody can be used for detection of Bcl-2 by Western blot at 1 to 4 μg/mL. Bcl-2 antibody can also detect Bcl-2 by immunohistochemistry at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Tested Application
E, WB, IHC, IF
Buffer
Bcl-2 Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. ZP-3335 - Bcl-2 Peptide
Storage
Bcl-2antibody can be stored at 4ËšC for three months and -20ËšC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Species Reactivity
H, M
Protein GI Number
20072668
Protein Accession Number
AAH27258
Reference

  • Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
  • Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.
  • Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron. 2004; 39:1125-35.
  • Hockenbery D, Nunez G, Milliman C, et al. Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature 1990; 348:334-6.]

 


Add to Cart:

Website by: san diego web designer