Aven Rabbit Polyclonal Antibody

$315.00

APO-2417

Background

Aven Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. Aven is a recently discovered protein that blocks apoptosis induced by Apaf-1 and caspase-9. It is thought that Aven functions by binding to Bcl-xL, an antiapoptotic member of the Bcl-2 family, and to Apaf-1, possibly interfering with the ability of Apaf-1 to self-associate, suggesting that Aven impedes Apaf-1-mediated caspase activation. Higher levels of Aven mRNA are seen in patients with acute leukemia than in control patients, suggesting that Aven may be useful as a prognostic indicator in leukemia patients.

 

Description
Aven Antibody
Western blot analysis of Aven in Raji cell lysate with Aven antibody at 1 μg/mL in (A) the presence and (B) the absence of blocking peptide.
Aven Antibody
Immunohistochemistry of Aven in human spleen tissue with Aven antibody at 5 μg/mL.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Specifications

Additional Names
Aven Antibody: Apoptosis caspase activation inhibitor
User Note
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgG
Immunogen
Aven antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human Aven.
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid 

Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal Aven Antibody.
Purification
Aven Antibody is affinity chromatography purified via peptide column.
Host
Aven antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.
Application
Aven antibody can be used for detection of Aven by Western blot at 1 μg/mL. Despite its predicted molecular weight, Aven often migrates at 55 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Tested Application
E, WB, IHC, IF
Buffer
Aven Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. ZP-2417 - Aven Peptide
Storage
Aven antibody can be stored at 4ËšC for three months and -20ËšC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Species Reactivity
H, M, R
Protein GI Number
9966841
Protein Accession Number
NP_065104
Reference

  • Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
  • Chau BN, Cheng EH-Y, Kerr DA, et al. Aven, a novel inhibitor of caspase activation. Binds Bcl-xL and Apaf-1. Mol. Cell 2000; 6:31-40.
  • Paydas S, Tanriverdi K, Yavuz S, et al. Survivin and aven: two distinct antiapoptotic signals in acute leukemias. Ann. Oncology 2003; 14:1045-50.]

 


Add to Cart:

Website by: san diego web designer