Apaf1 Rabbit Polyclonal Antibody




Apaf1 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1). Apaf1 binds to cytochrome c (Apaf-2) and caspase-9 (Apaf-3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 is ubiquitously expressed in human tissues.


Apaf1 Antibody
Western blot analysis of Apaf1 in K562 cell lysate with Apaf1 antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.
Apaf1 Antibody
Immunohistochemistry of Apaf1 in human heart tissue with Apaf1 antibody at 1 μg/mL.














Additional Names
Apaf1 Antibody: Apaf1, Apaf-1
User Note
Optimal dilutions for each application to be determined by the researcher.
Molecular Weight
115 - 130 kDa
This product is for research use only.
Physical State: Liquid 

Concentration: 1 mg/mL
This is a polyclonal Apaf1 Antibody.
Apaf1 Antibody is affinity chromatography purified via peptide column.
APAF1 antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.
Apaf1 antibody was raised against a peptide corresponding to amino acids near the amino terminus of human Apaf1. The sequences of the immunogenic peptide are identical between human and mouse.
Apaf1 antibody can be used for detection of Apaf1 by Western blot at 1 μg/mL. A 115 - 130 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL.
Tested Application
Apaf1 Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. ZP-2013 - Apaf1 Peptide
Apaf1 antibody can be stored at 4ËšC for three months and -20ËšC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Species Reactivity
H, M, R
Protein GI Number
Protein Accession Number

  • Zou H, Henzel WJ, Liu X, Lutschg A, Wang X. Apaf1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 1997;90:405-13
  • Cecconi F, Alvarez-Bolado G, Meyer BI, Roth KA, Gruss P. Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development. Cell 1998;94:727-37
  • Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES, Wang X. Cytochrome c and dATP-dependent formation ofApaf1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997;91:479-89
  • Hu Y, Benedict MA, Wu D, Inohara N, Nunez G. Bcl-XL interacts with Apaf1 and inhibits Apaf1-dependent caspase-9 activation. Proc Natl Acad Sci USA 1998;95:4386-91 (RD1299) ]


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