AES Antibody




AES Antibody: Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis. It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.


AES Antibody
Western blot analysis of AES in 293 cell lysate with AES antibody at (A) 2 and (B) 4 μg/mL.
AES Antibody
Immunocytochemistry of AES in 293 cells with AES antibody at 10 μg/mL.











Additional Names
AES Antibody: Amino-terminal enhancer of split, GRG, ESP1, TLE5
User Note
Optimal dilutions for each application to be determined by the researcher.
This product is for research use only.
Physical State: Liquid 

Concentration: 1 mg/mL
This is a polyclonal AES Antibody.
AES Antibody is affinity chromatography purified via peptide column.
AES antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.
AES antibody was raised against a 16 amino acid synthetic peptide from near the amino-terminus of human AES.
AES antibody can be used for the detection of AES by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Tested Application
AES Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. ZP-3609 - AES Peptide
AES antibody can be stored at 4ËšC for three months and -20ËšC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Species Reactivity
H, M
Protein GI Number
Protein Accession Number

  • Martin SS and Vuori K. Regulation of Bcl-2 proteins during anoikis and amorphosis. Biochim Biophys Acta. 2004; 1692:145-57.
  • Miyasaka H, Choudhury BK, Hou WE, et al. Molecular cloning and expression of mouse and human cDNA encoding AES and ESG proteins with strong similarity to Drosophila enhancer of split groucho protein. Eur. J. Biochem. 1993; 216:343-52.
  • Chen G and Courey AJ. Groucho/TLE family proteins and transcriptional repression. Gene 2000; 249:1-16.
  • Jan Y, Matter M, Pai J-t, et al. A mitochondrial protein, Bit1, mediates apoptosis regulated by integrins and groucho/TLE corepressors. Cell 2004; 116:751-762.]


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