ABL2 monoclonal antibody (M09), clone 5C6

Specification

Product Description:

Mouse monoclonal antibody raised against a partial recombinant ABL2.

Immunogen:

ABL2 (AAH65912, 743 a.a. ~ 842 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Immunogen Sequence (without GST):

KKTLGLRAGKPTASDDTSKPFPRSNSTSSMSSGLPEQDRMAMTLPRNCQR
SKLQLERTVSTSSQPEENVDRANDMLPKKSEESAAPSRERPKAKLLPRGA

Cross Reactivity:

Human

Isotype:

IgG3 Kappa

Storage Buffer:

In 1x PBS, pH 7.2

Storage Instruction:

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Quality Control Testing:

Antibody Reactive Against Recombinant Protein.

Western Blot detection against Immunogen (37 KDa) .

Applications

Western Blot (Cell lysate)

ABL2 monoclonal antibody (M09), clone 5C6 Western Blot analysis of ABL2 expression in K-562 ( Cat # L009V1 ). 

Western Blot (Recombinant protein)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunoperoxidase of monoclonal antibody to ABL2 on formalin-fixed paraffin-embedded human cerebellum. [antibody concentration 1.5 ug/ml] 

Immunofluorescence

Immunofluorescence of monoclonal antibody to ABL2 on HeLa cell. [antibody concentration 10 ug/ml] 

Sandwich ELISA (Recombinant protein)

Detection limit for recombinant GST tagged ABL2 is approximately 0.1ng/ml as a capture antibody. 

ELISA

Gene Information

Entrez GeneID:

27

GeneBank Accession#:

BC065912

Protein Accession#:

AAH65912

Gene Name:

ABL2

Gene Alias:

ABLL, ARG, FLJ22224, FLJ31718, FLJ41441

Gene Description:

v-abl Abelson murine leukemia viral oncogene homolog 2 (arg, Abelson-related gene)

Omim ID:

164690

Gene Ontology:

Hyperlink

Gene Summary:

This gene encodes a member of the Abelson family of nonreceptor tyrosine protein kinase. The protein is highly similar to the ABL1 protein, including the tyrosine kinase, SH2 and SH3 domains, and has a role in cytoskeletal rearrangements by its C-terminal F-actin- and microtubule-binding sequences. This gene is expressed in both normal and tumor cells, and is involved in translocation with the ETV6 gene in leukemia. Multiple alternatively spliced transcript variants encoding different protein isoforms have been found for this gene.

Other Designations:

Abelson murine leukemia viral (v-abl) oncogene homolog 2, Abelson-related gene protein, OTTHUMP00000033139, arg tyrosine kinase, v-abl Abelson murine leukemia viral oncogene homolog 2