Western Blot Analysis Custom Services

Zyagen offers complet Western blot custom services including extraction of total proteins from tissues or cells provided by Zyagen or customers, construction of premade Western blots, immunostaining blots with any antibody, quantitative analysis of immunostaining, and sendiing high quality digital images. We can construct blots with up to 20 samples including one prestained protein marker.

Protein Extraction Services:

  • Extraction and purification of protein from tissues provided by customers or Zyagen
  • Extraction and purification of protein from primary cell or cell lines provided by customers
  • Protein is extracted in any lysis buffer suggested by customers
  • To prevent protein degradation, protein lysis buffer is supplemented with a cocktail of 7 protease inhibitors.
  • Quantification of protein using highly specific and sensitive protein assays.
  • Quality of protein is tested by denatured SDS gel electrophoresis
  • Extracted protein is suitable for immediate use in reporter assays (e.g., luciferase, beta-galactosidase, chloramphenicol acetyl transferase), protein kinase assays (e.g., PKA, PKC, tyrosine kinase), immunoassays (e.g., Western blots, ELISAs, RIAs) and/or protein purification procedures, immunoprecipitation, SDS-PAGE, isoelectric focusing gels and SDS-capillary electrophoresis, enzymatic activity analysis, gel shifting assay, and protein-protein interaction.
  • Protein is provided in protein lysis buffer or denatured SDS sample buffer at any concentration suggested by customers
  • Protein is shipped on dry ice

Construction of Premade Western blotting Services:

  • Harvesting fresh tissues/cultured cells provided by Zyagen or customers.
  • Homogenization of tissues/cells in protein lysis buffer supplemented with a cocktail of 7 mammalian protease inhibitors.
  • Quantitation of  total protein using sensitive, specific and accurate protein assay.
  • Testing the purity and integrity of extracted proteins by gel electrophoresis.
  • Fractionation of protein (75 microgram) through large SDS-denatured polyacrylamide gel electrophoresis.
  • Transfer protein onto PVDF membranes.
  • Staining blots with reversible staining to check the sharpness of protein bands and efficiency of transfer.
  • Shipping blots at room temperature in sealed bags

Western Blot Analysis Services:

  • Extraction of total proteins from tissues/cells provided by Zyagen or customers
  • Constructions of Western blots using total proteins
  • Immunostaining blots with antibodies provided by customers using ultrasensative chemiluminescent ECL detection
  • Stripping antibodies from blots 
  • Reimmunostain blots with any house-keeping gene antibodies
  • Scanning protein bands on X-ray films
  • Quantitative analysis of immunostaining data
  • Sending high quality digital images of X-ray films on Microsoft PowerPoint

 

Western blot custom services ordering information:

Service I.D.  Title of the services Description
SWB-100 Extraction of Protein Lysates (total protein) Custom Services Extraction of total protein from tissues/cells provided by Zyagen or customers, quantitation of total protein by a sensitive accurate protein assay, and verification of purity and integrity of the extracted protein by SDS gel electrophoresis. Total proteins are shipped on dry ice. 
SWB-300 Western Blotting Custom Services

Fractionation of protein samples provided by Zyagen or customers through large SDS gels, transfer onto PVDF membranes, and staining blots with reversible staining to check the sharpness of protein bands and efficiency of transfer.  We routinely construct blots with up to 20 samples. Blots are sealed in bags and shipped at room temperature.

SWB-400 Western blot Analysis Custom Services Construction of blots with total proteins provided by Zyagen or customers, immunostaining with antibodies provided by customers, stripping blots and reimmunostaining with antibody of any house-keeping gene, scanning X-ray films, quantitative analysis of imunostaining data, and sending high quality digital images.

Services Ordering Information:

You can order services by phone, e-mail, fax or regular mail. See ordering information for more detail. Our specialized scientists will determine service cost according to the provided information and send you a formal quote with instructions and detailed information about experimental procedures by e-mail. The time frame of services is within 7 business days after receiving your order.

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