Northern Blot Analysis Custom Services

Zyagen offers Northern blot and Southern blot analysis services to extract total RNA, microRNA, purification of mRNA, isolation of genomic DNA and construct ready-to-hybridize Northern blots and southern blots. RNA/mRNA, DNA samples are extracted from tissues provided by Zyagen or customers. To ensure high quality blots, each sample whether provided by the customers or Zyagen  is subjected to rigorous quality control test before use. We can make blots with up to 20 tissues each plus one RNA or DNA marker.

Extraction of Total RNA, MicroRNA and Purification of mRNA Services:

  • Harvesting fresh tissues/cells provided by Zyagen or customers under RNase-free conditions.
  • Isolation of total RNA using classical Guanidinium thiocyanatephenol chloroform or column-based extraction methods.
  • Enrichmemt of microRNA from total RNA without using any purifictasion column .
  • Treatment of RNA with RNase-free DNase to remove residual DNA.
  • Purification of poly (A+) RNA from already extracted total RNA or directly from tissues.
  • Testing the purity and integrity of RNA by denatured gel electrophoresis and RT-PCR of beta-actin primers.
  • Dissolving RNA in water or any buffer suggested by customers
  • The extracted RNA is intact and of high quality and can be used in any application applied for gene expression analysis 

Extraction of Genomic DNA Services:

  • Harvesting fresh tissues/cells provided by Zyagen or customers under RNase-free conditions.
  • Isolation of DNA using classicalliquid-liquide extraction methods.
  • Treatment of DNA with DNase-free RNase to remove residual RNA.
  • Testing the purity and integrity of DNA by denatured gel electrophoresis and PCR of beta-actin primers.
  • Dissolving DNA in water or any buffer suggested by customers.
  • The extracted DNA is intact and of high quality and can be used in any application applied for gene expression analysis 

Construction of Northern Blots Services:

  • Isolation total RNA or mRNA from tissues provided by Zyagen or customers.
  • Fractionation of RNA (20 microgram of total RNA or 2 microgram of poly A+ RNA per lane) through formaldehyde-denatured agarose gel.
  • Transfer RNA onto nylon supercharged membranes.
  • Cross-linked RNA by UV light.
  • Staining blots with reversible staining (Ponceau) to check the sharpness of RNA bands and efficiency of transfer.
  • Sealed blots in bags and shipped at room temperature.

Construction of Southern Blots Services:

  • Isolation DNA from tissues provided by Zyagen or customers.
  • Fractionation of DNA (20 microgram of DNAper lane) through denatured agarose gels.
  • Transfer DNA onto supercharged membranes.
  • Cross-linked DNA by UV light.
  • Staining blots with reversible staining (Ponceau) to check the sharpness of DNA bands and efficiency of transfer.
  • Sealed blots in bags and shipped at room temperature.

Northern/Southern blotting custom services ordering information:

Service I.D.  Title of the services Description
SNB-100 Isolation of total RNA Custom Services Extraction of RNA from tissues/cells provided by Zyagen or customers, treatment RNA with RNase-free DNase, quantitation by sensitive assay, and verification of purity and integrity of the isolated RNA by denatured agarose gels and RT-PCR of beta actin.  Total RNA samples are shipped on dry ice.
SNB-200 Isolation of poly (A+) RNA  Custom Services Extraction of total RNA from tissues provided by Zyagen or customers, treatment RNA with RNase-free DNase, purification of poly (A+) RNA, quantitation by sensitive assay, and verification of purity and integrity of the purified mRNA by denatured agarose gels and RT-PCR of beta actin. Poly (A+) RNA samples are shipped on dry ice.
SNB-250 Isolation of Genomic DNA  Custom Services Extraction of Genomic DNA from tissues provided by Zyagen or customers, treatment DNA with DNase-free RNase, and verification of purity and integrity of the purified DNA by denatured agarose gels and PCR of beta actinDNA samples are shipped on dry ice, blue ice or at room temperature.
SNB-300 Northern blotting Custom services using total RNA and/or mRNA samples

Fractionation of total RNA or poly A+ RNA provided by Zyagen or customers through denatured agarose gel (20ug total RNA or 2ug poly A+ RNA per each sample per lane), and transfer onto nylon supercharged membranes. Each blot contains up to up 20 tissues. Blots are sealed in bag and shipped at room temperature.

SNB-350 Southern blotting Custom services using DNA samples Fractionation of DNA provided by Zyagen or customers through denatured agarose gel (20ug DNA per each sample per lane), and transfer onto supercharged membranes. Each blot contains up to up to 20 tissues. Blots are sealed in bag and shipped at room temperature.

Services Ordering Information:

You can order services by phone, e-mail, fax or regular mail. See ordering information for more detail. Our specialized scientists will determine service cost according to the provided information and send you a quotation with instructions and detailed information about experimental procedures  by e-mail. The time frame of services is within 7 business days of the date of receiving the order.

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