Immunohistochemistry Research Services

Single immunostaining:

Liver Mesothelium

Cerebellum

Cornea

Esophagus

Langerhans Islets

 

DRG

Myenteric Neurons

 

Double immunostaining in Langerhans Islets:

Antigen-1

Antigen-2

Overlapping

Zyagen offers immunohistochemistry services for pharmaceutical, biotechnology, academic, and federal research institutes using different methods of detection (immunofluorescence, alkaline phosphatase, and immunoperoxidase) on paraffin and frozen tissue sections. We routinely perform immunostaining using antibody provided by our client on tissue sections provided by Zyagen  or the clients. 

Assays are routinely run by highly trained scientists with more than 16 years of experience in immunohistochemical assay development and microscopic cell/tissue imaging analysis.

Complete immunohistochemistry (IHC) custom services include:

  • Optimization of tissue fixation process: This includes selection of suitable fixative agent and determine the optimal  incubation time with the fixative.
  • Optimization of antigen retrieval conditions: We are using detergent, enzymatic, or microwave retrieval methods to expose target antigen to the antibody.
  • Titration of  the primary antibody concentration and adjustment of the experimental conditions of the immunostaining.
  • Interpretation of  immunostaining results by our experts scientists.
  • Image and quantitative analysis of immunostaining if requested by the investigator. 
  • Sending high quality digital images.

 Providing investigators with a  report of the findings with  the microscopic images online. Color prints are available with an additional charge.

 

Service ID Title of the Service Description
SIH-200 Immunohistochemistry Custom Research Services Immunohistochemistry service using tissue sections provided by Zyagen or customers. Service include immunolocalization of the antigen of your interest in tissue sections of any rat, mouse, or other species using immunofluorescence, immunoperoxidase, or alkaline phosphatase detection methods, interpretation of immunostaining, and sending high quality digital images online. The primary antibody is provided by the customer.
SIH-400 Double Immunohistochemistry Custom Research Services Double immunohistochemistry service using tissue sections provided by Zyagen or customers. Service include immunolocalization of 2 or more antigens in the same tissue section using immunofluorescence method, interpretation of immunostaining, and sending high quality digital images online. The primary antibodies are provided by the customer.

Immunohistochemistry custom services ordering information:

You can order services by phone, e-mail, fax or regular mail. See ordering information for more detail. Our specialized scientists will determine service cost according to the provided information and send you a quotation with instructions and detailed information about experimental procedures  by e-mail or fax. The time frame of service is normally 10 business days from the date of receiving the order.

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