|Images of different tissue proteins used for construction of Western blots and immunostaining with 2 different antibodies. Click on each image for enlargment|
Plant tissue Western blots are made of high quality total proteins extracted from freshly harvested plant tissues. Each protein sample is subjected to rigorous quality control procedures before fractionation through large SDS gel.
Blots can be used to:
- Quickly assess specific protein expression across different plant tissues..
- Determine size and relative abundance of protein in tissues.
- Ready-to-use Western blots from high quality tissue lysates that are difficult to obtain because of size, high-protease tissue content, or anatomical complexity of the tissues which requires knowledge, experience, and technical skill to dissect the areas of interest freely of any surrounding tissues.
- Saving labor by eliminating tedious and time-consuming laboratory procedures (harvesting and homogenization of tissues, protein extraction, quantification, gel electrophoresis, and blotting).
- High standards of quality control
- Economic, using multiple tissues at a cost much lower than it would take to do in your laboratory. Think to labor and cost of laboratory materials, supplies and animals (purchasing, transportation, housing, disposable of carcasses and waste materials).
- Reusable for at least two times, just strip and use with other antibody.
Every step of preparation of blots, from harvesting tissues and extraction of protein to blotting is carefully monitored to ensure the superior quality and performance.
- Blots are made from high quality tissue lysates that is homogenized in a protein lysis buffer containing cocktail of 7 mammalian protease inhibitors. The quality of protein as indicated by the absence of smear (no degradation) and sharpness and resolution of protein bands are verified by denatured SDS-PAGE with Coomassie blue staining.
- The efficiency of transfer is routinely checked by staining protein on membranes with reversible Ponceau staining.
- The integrity of the blotted protein is tested by immunostaining of b-actin.
The total protein samples (75ug each ) are freshly extracted from plant leaves, boiled in SDS sample buffer, fractionated though 12.5% SDS-denaturing large polyacralamide gel eletrophoresis (SDS PAGE), and blotted onto PVDF membranes. Each blot contains up to plant samples plus one prestained protein molecular weight standards. Each blot is shipped in a sealed bag at room temperature and is good for one year if properly stored dry at 4oC in a sealed bag with descant.
Custom Services: Zyagen also offers custom services to make ready-to-use Western blots of any plant tissue specifically prepared to meet your applications, contact technical support at firstname.lastname@example.org or at 858 546 0720 for more information.
|Lane#||Panel# 1||Panel# 2||Panel# 3|
|Premade Tissues Western Blot||
Custom Made WB,
any 5 tissues of Panel# 1
Custom Made WB,
any 10 tissues of Panel# 1
|Cat.#: LW-MT-1||Cat.#: LW-CUS-1||Cat.#: LW-CUS-2|
|M||Protein Marker||Protein Marker||Protein Marker|
|1||Arabidopsis||Tissue 1||Tissue 1|
|2||Barley||Tissue 2||Tissue 2|
|3||Beans||Tissue 3||Tissue 3|
|4||Corn||Tissue 4||Tissue 4|
|5||Cotton||Tissue 5||Tissue 5|