Mouse C57 Tissue Panel WB

 
Images of different tissue proteins used for construction of Western blots and immunostaining with 2 different antibodies. Click on each image for enlargment
Mouse C57BL/6 tissue Western blots are made of high quality total proteins extracted from freshly harvested C57BL/6 mouse strain tissues. Each protein sample is subjected to rigorous quality control procedures before fractionation through large SDS gel. 
 
Blots can be used to:
  • Quickly assess specific protein expression across different tissues.
  • Determine size and relative abundance of protein  in different tissues.
Benefits:
  • Ready-to-use Western blots from high quality tissue lysates that are difficult to obtain because of size, high-protease tissue content, or anatomical complexity of the tissues which requires knowledge, experience, and technical skill to dissect the areas of interest freely of any surrounding tissues.
  • Saving labor by eliminating tedious and time-consuming laboratory procedures (harvesting and homogenization of tissues, protein extraction, quantification, gel electrophoresis, and blotting).
  • High standards of quality control
  • Economic, using multiple tissues at a cost much lower than it would take to do in your laboratory. Think to labor and cost of laboratory materials, supplies and  animals (purchasing, transportation, housing, disposable of carcasses and waste materials).
  • Reusable for at least two times, just strip and use with other antibody.
Quality control:
Every step of preparation of blots, from harvesting tissues and extraction of protein to blotting is carefully monitored to ensure the superior quality and performance.
  • Blots are made from high quality tissue lysates that is homogenized in a protein lysis buffer containing cocktail of 7 mammalian protease inhibitors. The quality of protein as indicated by the absence of smear (no degradation) and sharpness and resolution of protein bands are verified by denatured SDS-PAGE with Coomassie blue staining.
  • The efficiency of transfer is routinely checked by staining protein on membranes with reversible Ponceau staining.
  • The integrity of the blotted protein is tested by immunostaining of b-actin.
Description:
The total protein samples  (75ug each ) are freshly extracted from tissues, boiled in SDS sample buffer, fractionated though 12.5% SDS-denaturing large polyacralamide gel eletrophoresis (SDS PAGE), and blotted onto PVDF membranes.  Each blot contains up to 19 samples plus one prestained protein molecular weight standards. Each blot is shipped in a sealed bag at room temperature and is good for one year if properly stored dry at 4oC in a sealed bag with descant.
 
Custom Services: Zyagen also offers custom services to make ready-to-use Western blots of any set of tissues of any species specifically prepared to meet your applications, contact technical support at zinfo@zyagen.com or at 858 546 0720  for more information.
 

Mouse C57BL/6 Tissue Western Blots:

Lane  #
Panel# 1
Panel# 2
Panel# 3
Panel# 4
Panel# 5
Panel# 6
Panel# 7
Panel# 8
Panel# 9
Premade WB, Major Tissues
Premade NB,   Minor Tissues
Premade WB,   Neuronal Tissues
Premade WB, Digestive Tissues
Premade WB, Reproductive Tissues
Premade WB, Circulatory, Respiratory, Urinary, Immune Tissues
Custom Made WB, any 10 tissues of only Panel# 1
Custom Made WB, any 10 tissues of  Panel# 1 to 6
Custom Made WB, any 15 tissues of Panel# 1 to 6
MW-MT-1-C57
MW-MT-2-C57
MW-MT-3-C57
MW-MT-4-C57
MW-MT-5-C57
MW-MT-6-C57
MW-CUS-1-C57
MW-CUS-2-C57
MW-CUS-3-C57
M
Protein Marker
Protein Marker
Protein Marker
Protein Marker
Protein Marker
Protein Marker
Protein Marker
Protein Marker
Protein Marker
1
Brain
Pituitary
Brain, whole
Esophagus
Ovary
Heart
Tissue 1
Tissue 1
Tissue 1
2
Liver
Thyroid
Cerebellum
Stomach
Uterus
Atrium
Tissue 2
Tissue 2
Tissue 2
3
Pancreas
Adrenal
Hippocampus
Duodenum
Cervix
Ventricle
Tissue 3
Tissue 3
Tissue 3
4 Spleen Lymph Nodes Hypthalumus Jejunum Vagina Aorta Tissue 4 Tissue 4 Tissue 4
5
Heart
Eye
Thalamus
Ileum
Testis
Trachea
Tissue 5
Tissue 5
Tissue 5
6
Lung
Adipose
Striatum
Colon
Epididymis
Lung
Tissue 6
Tissue 6
Tissue 6
7
Esophagus
Bone Marrow
Medulla
Cecum
S. Vesicles
Kidney
Tissue 7
Tissue 7
Tissue 7
8
Stomach
Bone
Pons
Rectum
Vas Deferens
Bladder
Tissue 8
Tissue 8
Tissue 8
9
Intestine
Blood cells
Cerebral Cortex
Liver
Prostate
Thymus
Tissue 9
Tissue 9
Tissue 9
10
Colon

Olfactory Bulb
Pancreas
Placenta
Spleen
Tissue 10
Tissue 10
Tissue 10
11
S. Muscles

Spinal Cord
Salivary
Mammary
Lymph Nodes


Tissue 11
12
Kidney







Tissue 12
13
Thymus







Tissue 13
14
Testis







Tissue 14
15
Ovary







Tissue 15
16
Uterus








17
Placenta








18
Mammary








19
Skin







 



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