Plant Tissue Northern Blot

 
Images of tissue total RNA used for construction of Northern blots and analysis with cDNA probes. Click on each image for enlargment
Plant tissue Northern blots are made of high quality total RNA samples extracted from different plant fresh tissues. Each RNA sample is subjected to rigorous quality control procedures before fractionation through large denatured agarose gels and blotting onto positively charged nylon membranes.
 Blots can be used with either  radioactive or nonisotopic-labeled probes to:
  • Quickly assess gene expression across plant tissues.
  • Determine size and relative abundance of transcripts in different tissues.
  • Identify alternative splice forms.
Benefits:
  • Ready-to-hybridize Northern blots of total RNA of  tissues that are difficult  to obtain because of size, high RNase content, size, and anatomical complexity which requires knowledge, experience, and technical skill to exactly dissect the area of interest freely of surrounding tissues.
  • Saving labor by eliminating tedious and time-consuming laboratory procedures (harvesting and homogenization of tissues, RNA extraction, RNA quantification, gel electrophoresis, and blotting).
  • High standards of quality control
  • Economic, using multiple tissues at a cost much lower than it would take to do in your laboratory. Think to the labor and cost of laboratory materials, supplies and  animals (purchasing, transportation, housing, disposable of carcasses and waste materials). 
  • Reusable for several times, just strip probe and re-hybridize with new one.
Quality control: Every step of preparation of blots, from harvesting tissues and extraction of RNA to the blotting, is carefully monitored to ensure the superior quality and performance.
  • Blots are made of total RNA that is treated with RNase-free DNase to remove residual DNA. The purity and integrity of RNA are tested by denaturing agarose gel electrophoresis.
  • The efficiency of transfer is checked by staining gels with reversible staining .
  • The integrity of the blotted RNA is tested by beta-actin specific probe.
Description: The freshly isolated total RNA samples (20ug each) are fractionated though large formaldehyde-denatured agarose gel eletrophoresis, blotted onto a positively charged nylon membranes, and immobilized by UV light. Blots are shipped in a sealed bag at room temperature and are good for 1 year if stored at 4oC in the sealed bags.
 
Custom Services: If you are interested in Northern blot constructed with RNA samples not listed below or required blots to be specifically prepared to meet your application, our custom service scientists can make it for you.   For more information, contact technical support at zinfo@zyagen.com or at 858 546 0720.

 

Lane# Panel# 1 Panel# 2 Panel# 3
Premade Tissues NB
Custom Made NB,
any 5 tissues of Panel# 1
Custom Made NB,
any 10 tissues of Panel# 1
Cat.#: LN-MT-1 Cat.#: LN-CUS-1 Cat.#: LN-CUS-2
M RNA Marker  RNA Marker   RNA Marker
1 Arabidopsis Tissue 1 Tissue 1
2 Barley Tissue 2 Tissue 2 
3 Beans Tissue 3 Tissue 3
4 Corn Tissue 4 Tissue 4
5 Cotton Tissue 5 Tissue 5
6 Flaxseed   Tissue 6
7 Oat   Tissue 7
8 Potato   Tissue 8
9 Rapseed   Tissue 9
10 Rice   Tissue 10
11 Soybean    
12 Sunflower    
13 Tobacco    
14 Tomato    
15 Wheat    



Catalog #:
Product Name
Size
Price

LN-MT-1
1 Blot
$1,100.00
 



LN-CUS-1
1 Blot
$750.00
 



LN-CUS-2
1 Blot
$950.00
 







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