Pig Tissue Northern Blot

 
Images of tissue total RNA used for construction of Northern blots and analysis with cDNA probes. Click on each image for enlargment
Pig tissue Northern blots are made of high quality total RNA samples extracted from pig fresh tissues. Each RNA sample is subjected to rigorous quality control procedures before fractionation through large denatured agarose gels and blotting onto positively charged nylon membranes.
 Blots can be used with either  radioactive or nonisotopic-labeled probes to:
  • Quickly assess gene expression across pig tissues.
  • Determine size and relative abundance of transcripts in different tissues.
  • Identify alternative splice forms.
Benefits:
  • Ready-to-hybridize Northern blots of total RNA of  tissues that are difficult  to obtain because of size, high RNase content, size, and anatomical complexity which requires knowledge, experience, and technical skill to exactly dissect the area of interest freely of surrounding tissues.
  • Saving labor by eliminating tedious and time-consuming laboratory procedures (harvesting and homogenization of tissues, RNA extraction, RNA quantification, gel electrophoresis, and blotting).
  • High standards of quality control
  • Economic, using multiple tissues at a cost much lower than it would take to do in your laboratory. Think to the labor and cost of laboratory materials, supplies and  animals (purchasing, transportation, housing, disposable of carcasses and waste materials). 
  • Reusable for several times, just strip probe and re-hybridize with new one.
Quality control: Every step of preparation of blots, from harvesting tissues and extraction of RNA to the blotting, is carefully monitored to ensure the superior quality and performance.
  • Blots are made of total RNA that is treated with RNase-free DNase to remove residual DNA. The purity and integrity of RNA are tested by denaturing agarose gel electrophoresis.
  • The efficiency of transfer is checked by staining gels with reversible staining .
  • The integrity of the blotted RNA is tested by beta-actin specific probe.
Description: The freshly isolated total RNA samples (20ug each) are fractionated though large formaldehyde-denatured agarose gel eletrophoresis, blotted onto a positively charged nylon membranes, and immobilized by UV light. Blots are shipped in a sealed bag at room temperature and are good for 1 year if stored at 4oC in the sealed bags.
 
Custom Services: If you are interested in Northern blot constructed with RNA samples not listed below or required blots to be specifically prepared to meet your application, our custom service scientists can make it for you.   For more information, contact technical support at zinfo@zyagen.com or at 858 546 0720.

 

Lane# Panel# 1 Panel# 2 Panel# 3
Premade Tissues NB
Custom Made NB,
any 10 tissues of Panel# 1
Custom Made NB,
any 15 tissues of Panel# 1
Cat.#: PN-MT-1 Cat.#: PN-CUS-1 Cat.#: PN-CUS-2
M RNA Marker  RNA Marker   RNA Marker
1 Brain Tissue 1 Tissue 1
2 Liver Tissue 2 Tissue 2 
3 Pancreas Tissue 3 Tissue 3
4 Spleen Tissue 4 Tissue 4
5 Heart Tissue 5 Tissue 5
6 Lung Tissue 6 Tissue 6
7 Esophagus Tissue 7 Tissue 7
8 Stomach Tissue 8 Tissue 8
9 Intestine Tissue 9 Tissue 9
10 Colon Tissue 10 Tissue 10
11 S. Muscles   Tissue 11
12 Kidney   Tissue 12
13 Thymus   Tissue 13
14 Testis   Tissue 14
15 Ovary   Tissue 15
16 Uterus    
17 Spinal Cord    
18 Tongue    
19 Skin    



Catalog #:
Product Name
Size
Price

PN-MT-1
1 Blot
$850.00
 



PN-CUS-1
1 Blot
$650.00
 



PN-CUS-2
1 Blot
$750.00
 







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