Rat Tissue Panel Northern Blots

 
Images of tissue total RNA used for construction of Northern blots and analysis with cDNA probes. Click on each image for enlargment
Zyagen offer Northern blots made of a wide variety of Sprague Dawley rat tissues (neuronal, digestive, reproductive, circulatory, respiratory, urinary, immune, and mixed tissue panels). Blots are made of high quality total RNA samples which are subjected to rigorous quality control procedures before fractionation through large denatured agarose gels and blotting onto positively charged nylon membranes. .
 Blots can be used with either radioactive or nonisotopic-labeled probes to:
  • Quickly assess gene expression across rat tissues.
  • Determine size and relative abundance of transcripts in different tissues.
  • Identify alternative splice forms.
Benefits:
  • Ready-to-hybridize Northern blots of total RNA of  tissues that are difficult  to obtain because of size, high RNase content,  and anatomical complexity which requires knowledge, experience, and technical skill to exactly dissect the area of interest freely of surrounding tissues.
  • Saving labor by eliminating tedious and time-consuming laboratory procedures (harvesting and homogenization of tissues, RNA extraction, RNA quantification, gel electrophoresis, and blotting).
  • High standards of quality control
  • Economic, using multiple tissues at a cost much lower than it would take to do in your laboratory. Think to the labor and cost of laboratory materials, supplies and  animals (purchasing, transportation, housing, disposable of carcasses and waste materials). 
  • Reusable for several times, just strip probe and re-hybridize with new one.
Quality control:
Every step of preparation of blots, from harvesting tissues and extraction of RNA to the blotting, is carefully monitored to ensure the superior quality and performance.
  • Blots are made of total RNA that is treated with RNase-free DNase to remove residual DNA. The purity and integrity of RNA are tested by denaturing agarose gel electrophoresis.
  • The efficiency of transfer is checked by staining gels with reversible staining .
  • The integrity of the blotted RNA is tested by beta-actin specific probe.
Description: The freshly isolated rat tissue total RNA samples (20ug each) are fractionated though large formaldehyde-denatured agarose gel eletrophoresis, blotted onto a positively charged nylon membranes, and immobilized by UV light. Blots are shipped in a sealed bag at room temperature and are good for 1 year if stored at 4oC in the sealed bags.
 
Custom Services:  If you are interested in Northern blot constructed with any rat RNA tissue of any strain or required blots to be specifically prepared to meet your application, our custom service scientists can make it for you.   For more information, contact technical support at zinfo@zyagen.com or at 858 546 0720.

 Rat Tissue Northern Blots:

Lane  #
Panel# 1
Panel# 2
Panel# 3
Panel# 4
Panel# 5
Panel# 6
Panel# 7
Panel# 8
Panel# 9
Premade NB, Major Tissues
Premade NB,   Minor Tissues
Premade NB,   Neuronal Tissues
Premade NB, Digestive Tissues
Premade NB, Reproductive Tissues
Premade NB, Circulatory, Respiratory, Urinary, Immune Tissues
Custom Made NB, any 10 tissues of only Panel# 1
Custom Made NB, any 10 tissues of  Panel# 1 to 6
Custom Made NB, any 15 tissues of Panel# 1 to 6
RN-MT-1
RN-MT-2
RN-MT-3
RN-MT-4
RN-MT-5
RN-MT-6
RN-CUS-1
RN-CUS-2
RN-CUS-3
M
RNA Marker
RNA Marker
RNA Marker
RNA Marker
RNA Marker
RNA Marker
RNA Marker
RNA Marker
RNA Marker
1
Brain
Pituitary
Brain, whole
Esophagus
Ovary
Heart
Tissue 1
Tissue 1
Tissue 1
2
Liver
Thyroid
Cerebellum
Stomach
Uterus
Atrium
Tissue 2
Tissue 2
Tissue 2
3
Pancreas
Adrenal
Hippocampus
Duodenum
Cervix
Ventricle
Tissue 3
Tissue 3
Tissue 3
4 Spleen Lymph Nodes Hypthalumus Jejunum Vagina Aorta Tissue 4 Tissue 4 Tissue 4
5
Heart
Eye
Thalamus
Ileum
Testis
Trachea
Tissue 5
Tissue 5
Tissue 5
6
Lung
Adipose
Striatum
Colon
Epididymis
Lung
Tissue 6
Tissue 6
Tissue 6
7
Esophagus
Bone Marrow
Medulla
Cecum
S. Vesicles
Kidney
Tissue 7
Tissue 7
Tissue 7
8
Stomach
Bone
Pons
Rectum
Vas Deferens
Bladder
Tissue 8
Tissue 8
Tissue 8
9
Intestine
Blood cells
Cerebral Cortex
Liver
Prostate
Thymus
Tissue 9
Tissue 9
Tissue 9
10
Colon

Olfactory Bulb
Pancreas
Placenta
Spleen
Tissue 10
Tissue 10
Tissue 10
11
S. Muscles

Spinal Cord
Salivary
Mammary
Lymph Nodes


Tissue 11
12
Kidney







Tissue 12
13
Thymus







Tissue 13
14
Testis







Tissue 14
15
Prostate







Tissue 15
16
Ovary








17
Placenta








18
Mammary








19
Skin







 



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