Pregnancy-staged Sprague Dawley rat whole embryo, placenta, and mammary gland tissue Northern blots are made of high quality total RNA, which is subjected to rigorous quality control procedures. RNA is extracted from whole embryo, placenta, and mammary gland of pregnant mice. Pregnancy is precisely determined by observation of vaginal plug. Plug date is considered day one of gestation.
Blots allow you to quantify changes in the gene expression in embryo, placenta, and mammary gland at different stages of pregnancy.
Every step of preparation of blots, from harvesting tissues and extraction of RNA to the blotting, is carefully monitored to ensure the superior quality and performance.
- Blots are made of total RNA that is treated with RNase-free DNase to remove residual DNA. The purity and integrity of RNA are tested by denaturing agarose gel electrophoresis.
- The efficiency of transfer is checked by staining gels with reversible staining .
- The integrity of the blotted RNA is tested by beta-actin specific probe.
Description: The freshly isolated rat tissue total RNA samples (20ug each) are fractionated though large formaldehyde-denatured agarose gel eletrophoresis, blotted onto a positively charged nylon membranes, and immobilized by UV light. Blots are shipped in a sealed bag at room temperature and are good for 1 year if stored at 4oC in the sealed bags.
Custom Services: If you are interested in Northern blot of in rat embryo, placenta, and mammary gland of any strain or required blot to be specifically prepared to meet your application, our scientists can make it for you. For more information, contact technical support at firstname.lastname@example.org or at 858 546 0720.
Rat Embryo, Placenta, and Mammary Northern Blots:
|Lane#||Whole Embryo NB||Placenta NB||Mammary Gland NB|
|Cat.#: RN-104||Cat.#: RN-413||Cat.#: RN-414|
|M||RNA Marker||RNA Marker||RNA Marker|
|1||Embryo, E12||Pregnancy, E12||Pregnancy, E12|
|2||Embryo, E13||Pregnancy, E13||Pregnancy, E13|
|3||Embryo, E14||Pregnancy, E14||Pregnancy, E14|
|4||Embryo, E15||Pregnancy, E15||Pregnancy, E15|
|5||Embryo, E16||Pregnancy, E16||Pregnancy, E16|
|6||Embryo, E17||Pregnancy, E17||Pregnancy, E17|
|7||Embryo, E18||Pregnancy, E18||Pregnancy, E18|
|8||Embryo, E19||Pregnancy, E19||Pregnancy, E19|
|9||Embryo, E20||Pregnancy, E20||Pregnancy, E20|