Zyagen provides high quality ready to use cytoplasmic protein of wide variety of normal healthy chicken tissues that might difficult to obtain because of size, anatomical complexity, or high protease activity. Tissues are freshly dissected and homogenized in modified protein lysis buffer supplemented with a cocktail of 7 protease inhibitors to minimize proteolysis.
Quality Control:The quality of cytoplasmic protein as indicated by the absence of smear (no degradation) and sharpness and resolution of the protein bands are verified by denatured SDS-PAGE with Coomassie blue staining. Protein is also validated by Western Blot using specific cytoplasmic protein biomarker, GAPDH or beta-actin.
Applications: Cytoplasmic protein is ready to immediate use for studying cytoplasmic soluble proteins. Cytoplasmic protein is ideal for many downstream applications such as Western blotting, protein assays, enzyme-activity assays, gel electrophoresis, immunoprecipitation, protein-protein interaction analysis, and tissue specific expression identification.
Packing/Shipping/Storage:The extracted protein is provided in vials at a concentration of 2-3mg/ml and shipped on dry ice. Protein is stable for one year if stored properly at -70oC.
Custom Services:If you are interested in any cytoplasmic protein not listed in Zyagen website, please contact technical service at email@example.com for availability.
MSDS and Certificate of Analysis in PDF files: Contact Zyagen Technical Support at firstname.lastname@example.org