Zyagen Universal Reference Total Protein is a high quality standard for protein expression analysis prepared at industrial scales to minimize lot-to-lot variation. All tissues (organs) of several male and female Sprague Dawley rats are freshly dissected, cut in very small pieces, snap frozen, grinding to powder, and homogenized in protein lysis buffer supplemented with a cocktail of 7 protease inhibitors to minimize proteolysis.
The quality of protein as indicated by the absence of smear (no degradation) and sharpness and resolution of the protein bands are verified by denatured SDS-PAGE with Coomassie blue staining. The integrity of protein is tested by immunoblotting using specific beta-actin antibody.
The extracted protein is provided in 1.5 ml-tubes at a concentration of 5mg/ml and ready for immediate use in Western blotting, immunoprecipitation, SDS-PAGE, isoelectric focusing gels and SDS-capillary electrophoresis. Protein can also be provided in denatured SDS Sample buffer for only Western blotting use. Protein is stable for one year if stored properly at -70oC.
- High quality intact total protein prepared at industrial scales to minimize lot-to-lot variation.
- Consistent control for standard data set comparisons
- Extracted from all rat tissues for broad gene coverage.