Zyagen offers high quality ready to use total protein of human adult tissues which are obtained from certified tissue banks in USA. Tissues are freshly harvested by surgeons during surgical operation or from donors donated their tissues for scientific research. Tissues specimens are procured under strict approved protocols with informed consent and the utmost attention to issues of patient safety, anonymity and confidentiality.
Tissues are freshly dissected and homogenized in protein lysis buffer supplemented with a cocktail of 7 mamalian protease inhibitors to minimize proteolysis. The extracted proteins are precisely quantified, and packed in 1.5-ml tubes at a concentration of 5mg/ml. Protein is stable for one year if stored properly at -80oC.
The quality of protein as indicated by the absence of smear (no degradation) and sharpness and resolution of the protein bands is verified by denatured SDS-PAGE with Coomassie blue staining. The integrity of protein is tested by immunoblotting using specific beta-actin antibody.
The extracted protein is provided in vials at a concentration of 5mg/ml and and ready for immediate use in reporter assays (e.g., luciferase, beta-galactosidase, chloramphenicol acetyl transferase), protein kinase assays (e.g., PKA, PKC, tyrosine kinase), immunoassays (e.g., Western blots, ELISAs, RIAs) and/or protein purification procedures, immunoprecipitation, SDS-PAGE, isoelectric focusing gels and SDS-capillary electrophoresis, enzymatic activity analysis, gel shifting assay, and protein-protein interaction. Protein can also be provided in denatured SDS Sample buffer for only Western blotting use. Protein can also be provided in denatured SDS Sample buffer for Western blotting.
Custom Services: Zyagen also offers custom services for isolation of total protein of any tissue not listed below or of any other species. Contact Zyagen technical support for more information at firstname.lastname@example.org or at 858 546 0720.