Zyagen offers high quality ready to use total protein of cat tissues. Tissues are freshly dissected and homogenized in protein lysis buffer supplemented with a cocktail of 7 mamalian protease inhibitors to minimize proteolysis. The extracted proteins are precisely quantified, and packed in 1.5-ml tubes at a concentration of 5mg/ml. Protein is stable for one year if stored properly at -80oC.
The quality of protein as indicated by the absence of smear (no degradation) and sharpness and resolution of the protein bands is verified by denatured SDS-PAGE with Coomassie blue staining. The integrity of protein is tested by immunoblotting using specific beta-actin antibody.
The extracted protein is ready for immediate use in Western blotting, immunoprecipitation, SDS-PAGE, isoelectric focusing gels and SDS-capillary electrophoresis, enzymatic activity analysis, gel shifting assay, protein-protein interaction, and tissue specific expression.
Custom Services: Zyagen also offers custom services for isolation of total protein of any cat tissue not listed below or of any other species. Contact Zyagen technical support for more information at zinfo@zyagen.com or at 858 546 0720.