Rat Developmental Tissue Total Protein

Zyagen offers high quality ready to use total protein of several of Sprague Dawley rat developmental tissues (age related) including brain, heart, intestine, liver, lung, skin, skeletal muscles, stomach, testis, and ovary.

 Tissues are collected and homogenized in protein lysis buffer supplemented with a cocktail of 7 mammlian protease inhibitors to minimize proteolysis.

The extracted protein is precisely quantified, and packed in 1.5-ml tubes at a concentration of 5 mg/ml. Protein is stable for one year if stored at -80oC.

The quality of protein as indicated by the absence of smear (no degradation) and sharpness and resolution of the protein bands is verified by denatured SDS-PAGE with Coomassie blue staining. The integrity of protein is tested by immunostaining using specific beta-actin antibody.

The extracted protein is provided in vials at a concentration of 5mg/ml and and ready for immediate use in reporter assays (e.g., luciferase, beta-galactosidase, chloramphenicol acetyl transferase), protein kinase assays (e.g., PKA, PKC, tyrosine kinase), immunoassays (e.g., Western blots, ELISAs, RIAs) and/or protein purification procedures, immunoprecipitation, SDS-PAGE, isoelectric focusing gels and SDS-capillary electrophoresis, enzymatic activity analysis, gel shifting assay, and protein-protein interaction. Protein can also be provided in denatured SDS Sample buffer for only Western blotting use. Protein can also be provided in denatured SDS Sample buffer for Western blotting.











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