Match Set of RNA, DNA, protein
Zyagen offers high quality intact total RNA, DNA, and total protein simultaneously isolated of the same tissue of C57BL/6 mouse or Sprague Dawley rat. RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR analysis, rapid amplification of cDNA ends (RACE) and purification of mRNA for library construction. All RNA samples are treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80oC. The integrity of each RNA sample as indicated by intact 28S and 18S ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by spectrophotometer (A260/A280: 1.9-2.1). It is provided in RNase-free water, 1mM sodium citrate, or 0.1 mM EDTA at a concentration of 1 mg/ml.
DNA samples are suitable for PCR, Southern blot analysis, and DNA library construction. Samples are treated with DNase-free RNase to remove contaminant RNA and provided in 1.5-ml tubes at a concentration of 1mg/ml in TE buffer.
The quality of protein as indicated by the absence of smear (no degradation) and sharpness and resolution of the protein bands are verified by denatured SDS-PAGE with Coomassie blue staining. The integrity of protein is tested by immunoblotting using specific beta-actin antibody. Protein samples are provided in 1.5-ml tubes in protein lysis buffer supplemented with a cocktail of 7 protease inhibitors to minimize proteolysis at a concentration of 5mg/ml. Total protein samples can be used in in Western blotting, immunoprecipitation, SDS-PAGE, isoelectric focusing gels and SDS-capillary electrophoresis, enzymatic activity analysis, gel shifting assay, protein-protein interaction, and tissue specific expression.
Shipping and Storage: RNA, DNA, and protein set samples are shipped on dry ice and are stable for one year if stored properly at -70oC.
Custom Services: If you are interested in match set RNA, DNA, and protein of any rat tissue not listed below or tissues of other species, our research scientists can make it for you. Contact technical service at firstname.lastname@example.org for more information.