Mouse C57BL/6 Embryo Total RNA

Zyagen offers high quality pure intact total RNA of freshly harvested C57BL/6 mouse whole embryos at different stages of pregnancy. The pregnancy is precisely determined by observation of vaginal plug. Plug date is considered  day one of gestation. 

Total RNA is routinely extracted from embryos using the powerful classical guanidine isothiocyanate–phenol:chloroform extraction method which allows the rapid isolation of total RNA including microRNAs. RNA is treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80oC. The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by spectrophotometer (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR.

RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction.

Total RNA sample is routinely provided in RNase-free water at a concentration of 1 mg/ml and shipped on dry ice. Sample can also be provided in 1mM sodium citrate, 0.1 mM EDTA, or any other RNA storage buffer.

Custom Services: Zyagen also offers custom services for isolation of total RNA and mRNA of any mouse embryo of any strain. Contact technical services for more information.




Catalog #:
Product Name
Size
Price

MR-104-10-C57
0.05mg
$141.00
 



MR-104-11-C57
0.05mg
$141.00
 



MR-104-12-C57
0.05mg
$141.00
 



MR-104-13-C57
0.05mg
$141.00
 



MR-104-14-C57
0.1mg
$141.00
 



MR-104-15-C57
0.1mg
$141.00
 



MR-104-16-C57
0.2mg
$141.00
 



MR-104-17-C57
0.2mg
$141.00
 



MR-104-18-C57
0.2mg
$141.00
 



MR-104-005-C57
5X0.025mg
$730.00
 



MR-104-009-C57
9x0.025mg
$930.00
 







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