|Total RNA extracted from different tissues of different species. Click on each image for Enlargement|
Zyagen offers high quality pure intact total RNA of leaves of large variety of plant species. Total RNA is routinely extracted from plant tissues usining classical liquid:liquid RNA extraction method and column purified. RNA is treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80oC. The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by spectrophotometer (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR.
RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction..
Total RNA sample is routinely provided in RNase-free water at a concentration of 1 mg/ml and shipped on dry ice. Sample can also be provided in 1mM sodium citrate, 0.1 mM EDTA, or any other RNA storage buffer.
Custom Services: Zyagen also offers custom services for isolation of total RNA and mRNA of any tissue of any plant or animal species. Contact technical services for more information.