Zyagen offers high quality pure intact total RNAof rat uterus at different stages of pregnancy. The pregnancy is precisely determined by observation of vaginal plug. Plug date is considered day one of gestation.
We are routinelly using classical guanidine thiocyanate/phenol:chloroform extraction methods to extract both large and microRNA from tissues. Total RNA samples are then treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80oC. The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by spectrophotometer (A260/A280: 1.9-2.1).
RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR analysis, rapid amplification of cDNA ends (RACE) and purification of mRNA for library construction.
Total RNA is provided in RNase-free water, 1mM sodium citrate, or 0.1 mM EDTA at a concentration of 1 mg/ml and shipped on dry ice.
Custom Services: Zyagen also offers custom services for isolation of total RNA and mRNA of uteruine tissues of any rat strain or any other species. Contact customer service for more information.