Coronal brain frozen sections: brains of CD1 (ICR) mice are sectioned in coronal (cross) oreintation at 14 brain regions/areas (as illustrated below).
Brains are snapped frozen in OCT without any fixative, sectioned in coronal orientation at a thickness of 7-10 microns. Sections are mounted on positively charged slides. Each slide contains 2 sections. Frozen sections are shipped in tightly closed slide boxes or slide mailers on dry ice.
Brain coronal frozen sections of mice perifused with 10% neutral buffered formalin or 4% paraformeldehyde are also available upon request.
Frozen sections are made under RNase- and protease-free conditions and are ideal for immunohistochemistry and in situ hybridization.