Sagittal brain frozen sections: brains of Sprague Dawley rat are snapped frozen in OCT without any fixative, sectioned in sagittal oreintation at midline of brains at a thickness of 7-10 microns. Sections contain major brain areas/regions from olfactory bulb to cerebellum. Sections are mounted on positively charged slides. Each slide contains 2 sections. Frozen sections are shipped in tightly closed slide boxes or slide mailers on dry ice.
Brain frozen sagittal sections of rats perifused with 10% neutral buffered formalin or 4% paraformeldehyde are also available upon request. Frozen sections are made under RNase- and protease-free conditions and are ideal for immunohistochemistry and in situ hybridization