Zyagen offers high quality Sprague Dawley rat whole embryo frozen sections at different stages of pregnancy from E12 to E20 in sagittal orientation. Pregnancy is precisely determined by observation of vaginal plug. Plug date is considered day one of gestation. Sagittal sections are made close to the midline of each embryo so most major embryonic tissues/organs are shown in each section. Embryos are snapped frozen in OCT blocks, sectioned at a thickness of 7-10microns and mounted on positively charged slides (2 sections per slide). Fixed mouse embryo frozen sections are also available upon request.
Frozen sections are shipped in tightly closed slide boxes or slide mailers on dry ice and are good for one year if properly stored.
Applications: Frozen tissue sections are prepared in RNase-, protease-free environment and are ideal for immunohistochemistry and in situ hybridization.
Custom Research Services: If you are interested in frozen sections of any rat embryo of any mouse strain or required tissue sections to be specifically prepared to meet your application, please contact technical support at email@example.com or at 858 546 0720 for more information.