Zyagen offers highly optimized alkaline phosphatase immunohistochemistry kits to detect cell surface and intracellular antigens in frozen or paraffin-embedded tissue sections mounted on slides, floating sections in culture plates, and immobilized cells with high sensitivity and low background. Fast red is used as a substrate of alkaline phosphatase for visualization of antigenic structures in the tissues.
High specificity and sensitivity
Standardization with high reproducible results
Each kit contains serum blocking buffer, alkaline phosphatase suppresser, antibody diluent, biotinylated secondary antibody (cross-absorbed and purified), streptavidin-alkaline phosphatase conjugate, substrate, hematoxylin, and mounting medium.
Kits are easy to use, provided with instructions for each step from preparation of tissue sections to mounting slides with coverslips. In addition to the immunostaining procedure, the user manual also contains detailed technical information about processing frozen and paraffin tissue sections for immunostaining, antigen retrieval, and trouble-shootings.